Composite

Part:BBa_K2675050:Design

Designed by: Esteban Lebrun   Group: iGEM18_Evry_Paris-Saclay   (2018-10-05)


sfGFP-LVAtag expression casette under the control of pAimX(full) promoter of phage phi3T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 187
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pAimX(full) promoter (BBa_K2675020) + custom made RBS (BBa_K2675016) + sfGFP-LVAtag coding sequence (BBa_K2675006) + the strong L3S2P56 Terminator (BBa_K2675030)

Source

DNA synthesis & Assembly

References

[1] Erez Z, Steinberger-Levy I, Shamir M, Doron S, Stokar-Avihail A, Peleg Y, Melamed S, Leavitt A, Savidor A, Albeck S, Amitai G, Sorek R. Communication between viruses guides lysis-lysogeny decisions. Nature (2017) 541, 488-493.